THE PILOT UNIT OF ICSN - EXCELLENCE IN THE FIELD OF BIOACTIVE MICROBIAL SECONDARY METABOLITES
Solid-phase extraction (SPE) has long been used as a sample pretreatment technique to trap and concentrate highly diluted compounds. SPE is used extensively in the environmental and medical fields for pollutant detection and toxicological diagnostics. SPE has also gained interest in the field of Microbial Secondary Metabolites (MSM). Thus, in addition to the recovery of target compounds from cultivation broth, resins have been used to extend the fermentation time and to enhance the production by trapping toxic and inhibitory compounds. This technique has been combined with micro-scale automation to increase the throughput of screening or diagnostic tasks. Various sorbents have been developed for SPE, including reversed-phase silica (RPS), carbon-based sorbents (CBS) and apolar polystyrene–divinylbenzene copolymers (PS-DVB). In the field of MSM, modern developments have been made using PS-DVB. This type of resin exhibits a high capacity for target organic compounds together with a low selectivity. PS-DVB resins are stable to pressure, temperature, shearing, pH and organic solvents. Their use was extensively optimized according to their specific area, porosity, particle size, contact duration and resin/medium proportion.

 

SPE

 

Bicyclic and tetracyclic diterpenes from a Trichoderma symbiont of Taxus baccata.
Adelin E, Servy C, Martin M-T, Arcile G, I. Iorga B, Pretailleau P, Bonfill M, Ouazzani J.
Phytochemistry, Volume 97, 2014, Pages 55–61.
solation and Characterization of Unusual Hydrazides from Streptomyces sp. Impact of the Cultivation Support and Extraction Procedure.
Géraldine Le Goff, Marie-Thérèse Martin, Bogdan I. Iorga, Emilie Adelin, Claudine Servy, Sylvie Cortial, Jamal Ouazzani.
Journal of Natural Products. 2013, volume 76, issue 2,pages 142–149.
Application of solid-phase extraction to agar-supported fermentation.
Géraldine Le Goff, Emilie Adelin, Sylvie Cortial, Claudine Servy, Jamal Ouazzani.
Bioprocess Biosyst Eng. 2013, 36,1285–1290.
Isolation and characterization of α,β-unsaturated γ-lactono-hydrazides from Streptomyces sp.
Géraldine Le Goff, Marie-Thérèse Martin, Claudine Servy, Sylvie Cortial, Philippe Lopes, Anne Bialecki, Jacqueline Smadja, Jamal Ouazzani.
Journal of Natural Products. Volume 75, Issue 5, 2012, Pages 915-919.
Isolation, structure elucidation and biological activity of metabolites from Sch-642305-producing endophytic fungus Phomopsis sp. CMU-LMA.
Emilie Adelin, Claudine Servy, Sylvie Cortial, Hélène Lévaique, Marie-Thérèse Martin, Pascal Retailleau, Géraldine Le Goff, Boonsom Bussaban, Saisamorn Lumyong, Jamal Ouazzani.
Phytochemistry, Volume 72, Issue 18, 2011, Pages 2406-2412.